Bioluminescent single photon bioreactor and performing absolute quantification of light-producing activity by enzymes

A method implemented by a system of one or more processors, the system being included in a bioluminescent single photon bioreactor and for performing absolute quantification of light-producing activity by enzymes by the bioluminescent single photon bioreactor, the method comprising:

• producing, by a pixel detector of the bioluminescent single photon bioreactor, a detector signal;
• receiving, by an analyzer of the bioluminescent single photon bioreactor, the detector signal;
• determining, by the bioluminescent single photon bioreactor, a time of arrival of the detector signal;
• determining, by the bioluminescent single photon bioreactor, an autocorrelation among detector signals for threshold times of arrival of photons at pixel detector according to:
• the following equation for a single emitter in the field of view of an objective of the bioluminescent single photon bioreactor:
• g
• (
• 2
• )
• (
• τ
• )
• =
• 1
• -
• e
• -
• τ
• T
• ,
• or
• g(2)(0) for the number of emitters N in the field of view (FOV) of the objective, such that:
• if g(2)(0) is 0, then one enzyme was in the field of view;
• and
• if g(2)(0) is not 0, then the number of emitters N is greater than 1, or noise is present in the detector signal.