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US 12,428,577 B2Granted
Methods of monitoring in vitro transcription of mRNA and/or post-in vitro transcription processes
AssigneeModernaTX, Inc.
InventorElliott Schmitt
Life of this patent
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Abstract
Methods of monitoring in vitro transcription of mRNA and/or post-in vitro transcription processes are provided. For example, methods of monitoring in vitro transcription of mRNA and/or post-in vitro transcription processes by acquiring one or more Raman spectra are provided.
Claims30
A method comprising the steps of:
- a) performing in vitro transcription of mRNA;
- b) acquiring one or more Raman spectra during in vitro transcription of mRNA;
- c) determining whether the in vitro transcription has reached a desired endpoint, whether the in vitro transcription is progressing at a desired rate, and/or whether one or more reaction conditions are as desired;
- and
- d) modifying one or more reaction conditions after the determining step.
2 more elements
A method comprising the steps of:
- a) performing in vitro transcription of mRNA;
- b) acquiring one or more Raman spectra during the in vitro transcription of mRNA;
- c) monitoring one or more reaction conditions and/or progression of the in vitro transcription;
- and
- d) optimizing one of more reaction conditions of a subsequent batch of in vitro transcription based on data generated from the monitoring step.
2 more elements
Dependencies are inferred from the claim text.