Systems for isolation and/or separation of products from production processes

A method of producing renewable diesel fuel, comprising:

• (a) culturing a population of microorganisms selected from microalgae, oleaginous yeast or fungi, in the presence of a fixed carbon source, wherein:
• (i) the microorganisms accumulate at least 10% of their dry cell weight as lipid;
• and (ii) the fixed carbon source is selected from the group consisting of glycerol, depolymerized cellulosic material, sucrose, molasses, glucose, arabinose, galactose, xylose, fructose, mannose, acetate, and any combination of the foregoing;
• (b) isolating lipid from the cultured microorganisms using a cross-flow filtration cassette;
• and
• (c) subjecting the isolated lipids to one or more chemical reactions to generate straight chain alkanes, wherein renewable diesel is produced,
• wherein the cross-flow filtration cassette comprises an array of sheet members of generally rectangular and generally planar shape, each having a top and a bottom surface, wherein the sheet members include in sequence in said array a first retentate sheet, a first filter sheet, a permeate sheet, a second filter sheet, and a second retentate sheet, wherein when a medium to be filtered flows across the filter sheets, solids or high-molecular-weight species of diameter larger than the filter sheet's pore size are retained in the retentate flow, and permeate species present in the medium diffuse through the filter sheets and enter the permeate sheet and permeate flow, wherein each of the sheet members in said array has at least one inlet basin opening at one end thereof, and at least one outlet basin opening at an opposite end thereof, with permeate passage openings at longitudinal side margin portions of the sheet members, wherein each of the first and second retentate sheets have a multiplicity of channel openings, extending longitudinally between the inlet and outlet basin openings of the sheets in the array, and the first retentate sheet and the second retentate sheet, respectively, are bonded to the first filter sheet and the second filter sheet about peripheral end and side portions thereof, and wherein, for each of the arrays, the inlet basin openings are in register with one another, the outlet basin openings are in register with one another, and the permeate passage openings are in register with one another, and the permeate passage openings of each of the retentate sheets are circumscribingly bonded to an adjacent filter sheet, with a central portion of each of the retentate sheets and adjacent filter sheets being unbonded to permit permeate contacting the retentate sheets to flow through the filter sheets to the permeate sheet.