Centrifuge system for separating cells in suspension

Apparatus comprising:

• a multi-use rotatable centrifuge bowl,
• wherein the bowl is rotatable about a vertically extending axis, and
• includes an axially centered cavity,
• a single use structure, wherein the single use structure
• is removably positionable in the axially centered cavity, and
• when positioned in the axially centered cavity is rotatable in operative engagement with the centrifuge bowl,
• wherein in an operative position the single use structure includes
• an upper disc shape portion,
• a continuous annular outer wall,
• wherein the upper disc shape portion and the continuous annular outer wall bound an interior area of the single use structure,
• wherein the continuous annular outer wall
• is in operative fluid tight connection with the upper disc shape portion adjacent to an upper end of the continuous annular outer wall,
• internally bounds a separation chamber within the interior area, wherein in the separation chamber cell culture is separated into cell centrate and cell concentrate responsive to rotation of the single use structure in operative engagement with the centrifuge bowl,
• wherein at least a portion of separation chamber has a vertically truncated cone shape with a smaller inside radius adjacent to a lower end of the separation chamber that is lower than the upper end,
• at least one vertically extending axially centered fluid tight tube, wherein the at least one axially centered fluid tight tube
• extends through the upper disc shape portion, and
• is operative to at least one of
• deliver cell culture into the interior area of the single use structure,
• deliver cell centrate out of the interior area of the single use structure, and
• deliver cell concentrate out of the interior area of the single use structure.

Apparatus comprising:

• a multi-use rotatable centrifuge bowl,
• wherein the centrifuge bowl is rotatable about a vertically extending axis, and includes a vertically extending axially centered cavity,
• wherein the vertically extending axially centered cavity has a larger radius in a cavity upper portion than in a cavity lower portion,
• a single use structure, wherein the single use structure
• is removably positionable in the vertically extending axially centered cavity through the cavity upper portion, and
• when positioned in the vertically extending axially centered cavity is rotatable in operative engagement with the centrifuge bowl,
• wherein in an operative position the single use structure includes
• an upper disc shape portion,
• a continuous outer annular outer wall,
• wherein the upper disc shape portion and the continuous outer annular wall are engaged in operative fluid tight relation and bound an interior area of the single use structure,
• wherein the interior area of the single use structure includes a separation chamber,
• wherein the separation chamber
• it is bounded upwardly by the upper disc shape portion and radially by an inner surface of the continuous outer annular wall,
• has a vertically truncated cone shape with a smaller inside radius adjacent to a lower end of the separation chamber that is disposed vertically away from the upper disc shape portion,
• wherein rotation of the single use structure and the centrifuge bowl is operative to cause cell culture to be separated into cell centrate and cell concentrate in the separation chamber,
• at least one vertically extending axially centered fluid tight tube, wherein
• the at least one axially centered fluid tight tube
• extends in the upper disc shape portion, and
• is operative to deliver cell concentrate from an uppermost and radially outermost area of the separation chamber, out of the interior area of the single use structure.