Methods and compositions for the diagnosis, prophylaxis and treatment of Lyme disease

A reagent useful for the diagnosis of Lyme borreliosis in a subject comprising:

• i) a fusion protein of at least one outer surface protein consisting of:
• a) a peptide consisting of SEQ ID NO:
• 14;
• b) a peptide consisting of at least 90% identity to a peptide of SEQ ID NO:
• 14;
• or
• c) a peptide variant consisting of a sequence different in one or more conservative amino acids to a peptide of SEQ ID NO:
• 14;
• and
• ii) at least one additional outer surface protein of:
• (d) a peptide of SEQ ID NO:
• 10;
• (e) a peptide of SEQ ID NO:
• 11;
• (f) a peptide of SEQ ID NO:
• 12;
• (g) a peptide of SEQ ID NO:
• 13;
• (h) a peptide of SEQ ID NO:
• 14;
• (i) a peptide of SEQ ID NO:
• 15;
• (j) a peptide of SEQ ID NO:
• 16;
• (k) a peptide of SEQ ID NO:
• 17;
• (l) a peptide of SEQ ID NO:
• 18;
• (m) a peptide having at least 90% identity to a peptide of any of SEQ ID NO:
• 10 to 18;
• or
• (n) a peptide variant differing in one or more conservative amino acids to a peptide of any of SEQ ID NO:
• 10-18,
• wherein the at least one sequence of i) is not the same as the at least one sequence of ii).