Method for screening personalized intestinal environment-improving material and composition therefor
AssigneeHem Pharma Inc.
InventorsSoyoung Park, Yosep Ji
Life of this patent
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Abstract
The present disclosure relates to a composition for screening an intestinal environment-improving material and a screening method using the composition, and according to the composition and the method of the present disclosure, it is possible to provide an effective analysis method for screening a microbiota-improving candidate material in a personalized manner by providing a method for verifying personalized probiotics, prebiotics, foods, health functional foods and drugs under in vitro conditions based on microbiota and microbiota metabolites.
Claims17
A method for screening an intestinal environment-improving composition, comprising:
- (a) mixing a sample obtained from a subject with medium for screening the intestinal environment-improving composition, and homogenizing the sample, thereby obtaining the sample in medium;
- (b) treating the sample in medium from step (a) with the intestinal environment-improving composition and culturing a mixture including the sample in medium and the intestinal environment-improving composition;
- and
- (c) analyzing the cultured mixture from step (b) to determine content or concentration of toxin, intestinal microbiota, microbiota-derived metabolite, or a mixture thereof in the culture thereof,
- wherein
- the medium for screening the intestinal environment-improving composition does not include protein and carbohydrate;
- and
- the medium for screening the intestinal environment-improving composition comprises L-cysteine.
A method for screening an intestinal environment-improving composition, comprising:
- (1) preparing a mixture of human or animal feces and a Pharmaceutical Meta-Analysis Screening (PMAS) medium;
- (2) dispensing identical quantities of the feces-PMAS medium mixture into culture plates in an anaerobic chamber;
- (3) screening each of candidate intestinal environment-improving compositions;
- (4) culturing the prepared plates under anaerobic conditions with temperature, humidity and motion similar to those of the intestinal environment to ferment and culture respective test groups;
- and
- (5) centrifuging the cultured respective test groups of step (4) to obtain supernatant;
- and
- (6) testing for the presence of toxic or undesirable substances comprising hydrogen sulfide, bacterial LPS, short-chain fatty acids in the supernatant, and/or microbiota.
Dependencies are inferred from the claim text.