Oncolytic virus expressing PD-1 binding protein and application of oncolytic virus
AssigneeShanghai Yuansong Biotechnology Co. Ltd.
InventorsKangjian Zhang, Xianlong Fang, Jinfa Gu, Xinyuan Liu
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Abstract
Provided is an oncolytic virus expressing a PD-1 binding protein, i.e., an oncolytic adenovirus comprising a fusion protein expressing a PD-1 single-chain antibody, wherein the fusion protein has a structure of S-VL-L-VH-Fc, S is an optional signal peptide sequence, VL is the light chain variable region of the PD-1 single-chain antibody, L is a flexible linker, VH is the heavy chain variable region of the PD-1 single-chain antibody and Fc is an immunoglobulin Fc fragment.
Claims18
An oncolytic virus comprising a nucleic acid encoding a PD-1 binding protein capable of inhibiting PD-1 activity, wherein the PD-1 binding protein is a fusion polypeptide comprising a PD-1 single-chain antibody and an immunoglobulin Fc fragment;
- and
- the fusion polypeptide has a structure of S-VL-L-VH-Fc;
- S is an optional signal peptide sequence, and the signal peptide sequence is MDMRVPAQLLGLLLLWLPGARC (SEQ ID NO:
- 14), or MDMRVPAQLLGLLLLWFPGSRC (SEQ ID NO:
- 16);
- VL is the light chain variable region of the PD-1 single-chain antibody, comprising CDR1 with sequence RAGONVONYLA (SEQ ID NO:
- 17), CDR2 with sequence NAQSLQT (SEQ ID NO:
- 18) and CDR3 with sequence QQYNSWPT (SEQ ID NO:
- 19);
- L is a flexible linker comprising or mainly consisting of Ala (A), Thr (T), Gly (G) and/or Ser(S);
- VH is the heavy chain variable region of the PD-1 single-chain antibody, comprising CDR1 with sequence GFSLSTSGT (SEQ ID NO:
- 20), CDR2 with sequence CWEDS (SEQ ID NO:
- 21) and CDR3 with sequence EDSGYFWFPY (SEQ ID NO:
- 22);
- and
- Fc is an immunoglobulin Fc fragment, wherein the Fc fragment is derived from the Fc fragment of IgG, IgA, IgD, IgE or IgM.
Dependencies are inferred from the claim text.