Display substrates and methods of manufacturing the same, display panels, and display apparatuses

A display substrate, comprising:

• a base substrate;
• a plurality of light-emitting structures provided on the base substrate;
• and
• a pixel definition layer provided on the base substrate and having a plurality of pixel openings, the pixel definition layer comprising a first definition layer and a second definition layer on the first definition layer, and the second definition layer being made of a conductive material, wherein the pixel definition layer further comprises a third definition layer located on the second definition layer and made of a hydrophobic material, and at least a portion of the second definition layer is exposed from the third definition layer,
• wherein each of the plurality of light-emitting structures comprises a first electrode, a light-emitting material, and a second electrode, the first electrode is provided on the base substrate and located in a respective pixel opening, the light-emitting material is provided on the first electrode, and second electrodes of the plurality of light-emitting structures are provided on the light-emitting material and are structurally integrated, and the second electrodes cover the pixel definition layer and are electrically connected with the second definition layer.