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US 12,223,651 B2Granted
Identifying nucleotides by determining phasing
AssigneeIllumina, Inc.
InventorsFrancisco Jose Garcia, Klaus Maisinger, Stephen Tanner, John A. Moon, Tobias Mann, Michael Lawrence Parkinson, Anthony James Cox, Haifang H. Ge
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Abstract
Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.
Claims18
A method for creating a template of nucleic acid cluster locations on a flow cell, comprising:
- obtaining fluorescent emission signals from nucleic acid clusters on the flow cell, wherein a nucleic acid cluster comprises a plurality of nucleic acid molecules attached to the flow cell, and wherein the plurality of the nucleic acid molecules comprise fluorescently labeled nucleotides;
- identifying a plurality of candidate cluster locations on the flowcell from the obtained fluorescent emission signals;
- and
- determining whether to keep a candidate cluster location, discard a candidate cluster location, or merge a candidate cluster location with another location based on the obtained fluorescent emission signals, thereby generating a template of cluster locations on the flow cell.
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Dependencies are inferred from the claim text.