Establishing fluidic connections between chromatography components

A clamp assembly comprising:

• a rail extending along the length and configured to receive a first fluidic assembly;
• and
• a carriage movably attachable to the rail such that the carriage moves along the rail, the carriage configured to receive a second fluidic assembly, the carriage including an actuator and a stop mechanism,
• wherein the stop mechanism is configured to selectively prevent and allow movement of the carriage relative to the rail, wherein the stop mechanism is configured to be independently operable from the actuator assembly, and wherein engaging the actuator is configured to simultaneously establish two fluid tight seals by:
• moving a chromatography column received by the clamp assembly relative to the rail to create a first fluid tight seal between the chromatography column and the first fluidic assembly, wherein the engaging the actuator is configured to operably move the first fluidic assembly into engagement with the chromatography column to create the first fluid tight seal, and
• moving the second fluidic assembly relative to the carriage body to create a second fluid tight seal between the second fluidic assembly and the chromatography column, wherein the engaging the actuator is configured to simultaneously push the chromatography column into the second fluid tight seal with the second fluidic assembly.

A method of establishing fluid tight seals comprising:

• receiving a first chromatography column by a clamp assembly;
• moving a carriage along a rail of the clamp assembly into a first position that corresponds to a length of the first chromatography column, the carriage including an actuator and a stop mechanism;
• preventing movement, by the stop mechanism of the carriage, relative to the rail at the first position;
• after the preventing movement, engaging the actuator of the carriage;
• by the engaging of the actuator, simultaneously establishing two fluid tight seals including establishing a first fluid tight seal between the first fluidic assembly and the first chromatography column, wherein the engaging the actuator is configured to operably move the first fluidic assembly into engagement with the chromatography column to create the first fluid tight seal, and establishing a second fluid tight seal between the second fluidic assembly and the first chromatography column, wherein the engaging the actuator is configured to simultaneously push the chromatography column into the second fluid tight seal with the second fluidic assembly.