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US 12,222,279 B2Granted
Flow cell device and bioreactor product monitoring system and method
AssigneeNirrin Technologies, Inc.
InventorsBryan A. Hassell, Aaron Delahanty
Life of this patent
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Abstract
A flowcell device, including a flow pathway and an optical subassembly, has a flowcell body that is continuous with a sample being analyzed and a temperature controlled surface. The flowcell body can be disposed between a thermalplate, actively regulated by a thermoelectric cooler, and an insulating member. The flowcell device can be employed in a bioreactor monitoring system.
Claims20
A flowcell device comprising:
- a light source;
- a flowcell body defining a flow pathway that includes a central cell;
- a thermal plate in thermal contact with the flowcell body;
- a Peltier device for controlling a temperature of the flowcell body via the thermal plate;
- and
- an optical subassembly, comprising the central cell and adjacent first and second optical windows and a light source and a detector for determining an absorption spectra of a sample in the central cell via the first and second optical windows,
- wherein, in the central cell, the flow pathway and a light pathway from a light source through the central cell are orthogonal to one another, and wherein the flow pathway comprises a U-shaped channel that includes the central cell.
4 more elements
An assembly comprising:
- a bioreactor containing cells and a culture media;
- an actively temperature-controlled thermal plate;
- an optical source;
- an optical detector;
- a flowcell device having a flow cell body defining a channel that includes a central cell;
- a pump and a connector, whereby the sample is flowed from the bioreactor by the pump through the connector to the channel that includes the central cell, whereby the pump, the connector, and the channel that includes the central cell are fluidically connected;
- wherein the flowcell body has an optical subassembly, comprising the central cell and adjacent first and second optical windows,
- wherein the flowcell body is held between an insulating member and the actively temperature-controlled thermal plate while an absorption spectra of the sample is acquired using the optical source and the optical detector.
5 more elements
An optofluidic system, comprising:
- a flowcell device having a flowcell body defining a flow pathway that includes a channel comprising a central cell;
- a bioreactor containing cells and a culture media;
- a pump and a connector, whereby the sample is flowed from the bioreactor with the pump through the connector to the channel comprising the central cell, whereby the pump, the connector, and the channel comprising the central cell are fluidically connected;
- an optical subassembly, comprising the central cell, a first optical window and a second optical window;
- a light source for directing light through the first optical window, the central cell and the second optical window;
- an optical signal detector;
- a thermal plate in contact with the flowcell body and supporting a thermoelectric cooler;
- a thermal controller;
- a sample flow controller;
- a light source controller and/or detector controller;
- and
- a computer configured for controlling the thermal controller, the sample flow controller and the light source and/or detector controller, wherein the computer is configured for instructing the thermal controller to maintain a temperature of the sample in the flow cell device while acquiring an absorption spectra of the sample using the light source and the optical signal detector.
9 more elements
Dependencies are inferred from the claim text.