Inter-cluster intensity variation correction and base calling

A non-transitory computer readable storage medium storing computer instructions that, when executed by at least one processor, cause a system to:

• access, for a target cluster, current intensities registered from the target cluster and other clusters for a current sequencing cycle;
• determine a set of current accumulated intensity correction parameters for the current sequencing cycle based on the current intensities;
• determine, for the target cluster and based on the set of current accumulated intensity correction parameters, a current amplification coefficient;
• correct next intensities registered for a next sequencing cycle based on the current amplification coefficient;
• and
• determine a base call for the target cluster at the next sequencing cycle based on the corrected next intensities.

A computer-implemented method comprising:

• accessing, for a target cluster, current intensities registered from the target cluster and other clusters for a current sequencing cycle;
• determining a set of current accumulated intensity correction parameters for the current sequencing cycle based on the current intensities;
• determining, for the target cluster and based on the set of current accumulated intensity correction parameters, a current amplification coefficient;
• correcting next intensities registered for a next sequencing cycle based on the current amplification coefficient;
• and
• determining a base call for the target cluster at the next sequencing cycle based on the corrected next intensities.