Reagent storage in an assay device

A multi-well assay plate comprising a plate body comprising a plurality of assay wells and one or more reagent spaces defined in said plate body, wherein the one or more reagent spaces are in fluidic communication with one or more surrounding wells of said plurality of wells, wherein

• (a) said one or more reagent spaces comprise a storage zone comprising a storage surface to which two or more surface-reagent complexes are bound and thereby confined to said storage zone, said two or more surface-reagent complexes comprising a first surface-reagent complex and a second surface-reagent complex, said first surface-reagent complex comprising:
• (i) a first reagent linked to a first targeting agent;
• and
• (ii) a first surface linked to a second targeting agent, and said second surface-reagent complex comprising:
• (iii) a second reagent linked to a third targeting agent;
• and
• (iv) a second surface linked to a fourth targeting agent,
• wherein said first reagent and said first surface and said second reagent and said second surface are linked, respectively, in said first and second surface-reagent complexes, via a selective releasable binding interaction between said first and second targeting agents and said third and fourth targeting agents, respectively, wherein said first reagent is released from said first surface-reagent complex by a first set of conditions that differ from a second set of conditions used to release said second reagent from said second surface-reagent complex;
• and
• (b) said one or more surrounding wells are configured to use at least one of said first and second reagents in an assay for an analyte of interest in a sample.

A multi-well assay plate comprising a plate body comprising (a) a plurality of assay wells comprising one or more use zones;

• and (b) one or more storage surfaces positioned in said plate body, wherein the one or more storage surfaces do not overlap with said one or more use zones, wherein the one or more storage surfaces are in fluidic communication with one or more wells of said plurality of wells, wherein
• (a) said one or more storage surfaces comprise two or more surface-reagent complexes bound to said one or more storage surfaces,
• said two or more surface-reagent complexes comprising a first surface-reagent complex and a second surface-reagent complex, said first surface-reagent complex comprising:
• (i) a first reagent linked to a first targeting agent;
• and
• (ii) a first surface linked to a second targeting agent, and said second surface-reagent complex comprising:
• (iii) a second reagent linked to a third targeting agent;
• and
• (iv) a second surface linked to a fourth targeting agent,
• wherein said first reagent and said first surface and said second reagent and said second surface are linked, respectively, in said first and second surface-reagent complexes, via a selective releasable binding interaction between said first and second targeting agents and said third and fourth targeting agents, respectively, wherein said first reagent is released from said first surface-reagent complex by a first set of conditions that differ from a second set of conditions used to release said second reagent from said second surface-reagent complex;
• and
• (b) said one or more use zones are each configured to use at least one of said first and second reagents in an assay for one or more analytes of interest in a sample.