Isolated organ evaluation and treatment

A method of treating an isolated organ with existing damage during isolated organ perfusion, wherein the isolated organ is determined during the method to be an isolated organ with existing damage based on comprising cells that have ruptured, the method comprising steps of:

• perfusing the isolated organ with a perfusate;
• measuring a concentration of a cell rupture marker in the perfusate during the perfusing of the isolated organ;
• comparing the concentration of the cell rupture marker in the perfusate to a reference value of the cell rupture marker, as the perfusing of the isolated organ continues, to identify the isolated organ as comprising cells that have ruptured;
• adding a tissue factor (TF) suppressor molecule to the perfusate, as the perfusing of the isolated organ continues, only after having identified the isolated organ as comprising cells that have ruptured;
• and
• further perfusing the isolated organ with the perfusate after the TF suppressor molecule has been added, thereby treating the isolated organ, wherein
• the cell rupture marker comprises one or more of double stranded DNA (dsDNA), mitochondrial DNA, or ribosomes;
• and
• the TF suppressor molecule comprises a cyclooxygenase (COX) inhibitor.