Sample analyzer and sample analysis method

A sample analyzer, comprising:

• a sampling apparatus configured to collect a blood sample;
• a sample preparation apparatus provided with a reaction chamber and a reagent supply portion, wherein the reaction chamber is configured to receive the blood sample collected by the sampling apparatus, and the reagent supply portion is configured to supply a hemolytic agent and a dye to the reaction chamber, so that the blood sample collected by the sampling apparatus is mixed with the hemolytic agent and the dye provided by the reagent supply portion in the reaction chamber to prepare a test sample liquid, and wherein the hemolytic agent is used to lyse red blood cells, and the dye is used to stain organelles in blood cells;
• an optical detection apparatus comprising a light source, a flow chamber and an optical detector, wherein the light source is configured to emit a light beam to irradiate the flow chamber, the flow chamber is configured to communicate with the reaction chamber, wherein particles in the test sample liquid are capable of passing through the flow chamber one by one, and the optical detector is configured to detect, in a single test, scattered light signals and fluorescence signals generated by the particles passing through the flow chamber after the particles are irradiated by the light beam;
• and
• a processor configured to perform the following steps:
• obtaining the scattered light signals and the fluorescence signals of the test sample liquid detected by the optical detection apparatus in the single test of the test sample liquid, wherein the scattered light signals comprise forward-scattered light signals and side-scattered light signals;
• obtaining a particle concentration of blast cells in the blood sample based on the side-scattered light signals and the fluorescence signals;
• and obtaining a particle concentration of nucleated red blood cells and/or a particle concentration of basophil granulocytes in the blood sample based on the forward-scattered light signals and the fluorescence signals, so as to obtain the particle concentration of blast cells and the particle concentration of nucleated red blood cells and/or the particle concentration of basophil granulocytes in the single test.