US 12,215,394 B2 · Molecular marker nicotine associated SNP 1 for identifying high or low nicotine content of tobacco and its kit as well as use thereof

Abstract

The present invention “A Molecular marker Nicotine Associated SNP 1 for identifying high or nicotine content of tobacco and its kit as well as use thereof” belongs to field of molecular biology technology. The molecular marker Nicotine Associated SNP 1 is a SNP Nitab4.5_0002539:95304 A/G at base No. 95304 of Genomic segment No. 0002539 in tobacco genome version of Nitab v4.5 Genome Scaffolds Edwards2017. The present invention can accurately identify and screen tobacco germplasm resources with high or low nicotine content, and the screened tobacco can be directly used for breeding new tobacco varieties without transgenic methods. At the same time, gene sequence where the SNP site is located can also significantly activate promoters of key enzyme genes in nicotine synthesis pathway.

Claims6

Dependent claims

A kit for identifying high or low nicotine content of tobacco, comprising a molecular marker Nicotine Associated SNP 1, wherein the molecular marker Nicotine Associated SNP 1 is a single nucleotide polymorphism SNP site Nitab4.5_0002539:95304 A/G at base No. 95304 of Genomic segment No. 0002539 in tobacco genome version of Nitab v4.5 Genome Scaffolds Edwards2017;

the SNP site is located at the 324th nucleotide of the amplicon sequence set forth in SEQ ID NO:
13, which is derived from the tobacco genome using primers set forth in SEQ ID NO:1 and SEQ ID NO:2, and wherein the kit further comprises a pair of specific primers for the molecular marker Nicotine Associated SNP 1 comprising SEQ ID NO:1 and SEQ ID NO:2.

A method for breeding tobacco varieties with high or low nicotine content, wherein tobaccos with high or low nicotine content from candidate tobaccos are screened by using a molecular marker Nicotine Associated SNP 1 for identifying high or low nicotine content of tobacco comprising a single nucleotide polymorphism (SNP) site Nitab4.5_0002539:95304 A/G at base No. 95304 of Genomic segment No. 0002539 in tobacco genome version of Nitab v4.5 Genome Scaffolds Edwards2017;

the SNP site is located at the 324th nucleotide of the amplicon sequence set forth in SEQ ID NO:13, which is derived from the tobacco genome using primers set forth in SEQ ID NO:1 and SEQ ID NO:2.

Dependencies are inferred from the claim text.

Facts

Application

17/534,204Filed 23 Nov 2021

Priority

CN 202111324714.410 Nov 2021

Published

US 2023/0203601 A129 Jun 2023

Granted

4 Feb 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae
A01H 1/04
Processes for modifying genotypes ; Plants characterised by associated natural traits: processes of selection involving genotypic or phenotypic…
A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 5/12
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: leaves

Molecular marker nicotine associated SNP 1 for identifying high or low nicotine content of tobacco and its kit as well as use thereof

Life of this patent

Molecular marker nicotine associated SNP 1 for identifying high or low nicotine content of tobacco and its kit as well as use thereof

Life of this patent

Abstract

Claims6