Nucleic acid sequence measurement device

A nucleic acid sequence measurement device that measures a target having a specific nucleic acid sequence by means of hybridization, a sample being a solution containing the target, the nucleic acid sequence measurement device comprising:

• a fluorescent probe with a fluorescent molecule added at a predetermined position;
• a quenching probe with a quenching molecule added at a predetermined position;
• and
• a substrate having a solid phase surface to which a base end of each of the fluorescent probe and the quenching probe is fixed,
• wherein one of the fluorescent probe and the quenching probe has a detection sequence having a nucleic acid sequence complementary to a nucleic acid sequence of the target,
• wherein the other one of the fluorescent probe and the quenching probe has a nucleic acid sequence complementary to a nucleic acid sequence of a part of the detection sequence (which will also be referred to as a complementary sequence) and a nucleic acid sequence from a base end of the complementary sequence on a side of the solid phase surface to the other base end is complementary to the part of the detection sequence,
• wherein the fluorescent probe or the quenching probe having the detection sequence has a longer nucleic acid sequence than the fluorescent probe or the quenching probe having the complementary sequence,
• wherein the base end of the fluorescent probe and the base end of the quenching probe are fixed to the solid phase surface so that a positional relationship in which fluorescence emitted by the fluorescent molecule is quenched by the quenching molecule close to the fluorescent molecule is established, and
• wherein the nucleic acid sequence from the base end of the complementary sequence on the side of the solid phase surface to the other base end is complementary to a nucleic acid sequence from a base end of the detection sequence on the side of the solid phase surface to a portion corresponding to the other base end of the complementary sequence.