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US 12,215,368 B2Granted
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
AssigneeCodexis, Inc.
InventorsXiang Yi, Oscar Alvizo, Ravi David Garcia, David Entwistle, Charlene Ching, James Nicholas Riggins
Life of this patent
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Abstract
The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Claims17
An engineered polypeptide having imine reductase activity and comprising an amino acid sequence with at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 4, wherein the tyrosine at the position corresponding to amino acid 222 of SEQ ID NO:
- 4 is substituted with another amino acid in the sequence of the engineered polypeptide.
Dependencies are inferred from the claim text.