Apparatus and method for establishment of induced pluripotent stem cells
AssigneesCanon Medical Systems Corporation, Canon Kabushiki Kaisha
InventorKatsuaki Deguchi
Life of this patent
Estimating the term…
Abstract
According to one embodiment, a method for the establishment of an induced pluripotent stem (iPS) cell includes a suspension liquid feeding step, an inducing factor feeding step, and an establishing step. The suspension liquid feeding step feeds a suspension liquid containing a target cell. The inducing factor feeding step feeds an inducing factor to a trap. The trap traps the target cell contained in the suspension liquid fed in the suspension liquid feeding step. The establishing step establishes an iPS cell by introducing the inducing factor, which has been fed to the trap, into the target cell trapped by the trap.
Claims16
A method for supplying a mixed liquid containing a mononuclear cell and an inducing factor for inducing reprogramming of the mononuclear cell to a container, the method comprising:
- a first step of sending a suspension liquid containing the mononuclear cell to a trap through a first flow channel in a first direction, the trap configured to trap the mononuclear cell contained in the suspension liquid sent in the first step, the trap being arranged in the first flow channel;
- and
- a second step of sending the inducing factor to the trap in a second direction opposite the first direction through the first flow channel, and sending the mononuclear cell trapped by the trap and the inducing factor to the container through a second flow channel branching off from the first flow channel at a branch position upstream of the trap in the first direction.
A liquid supplying apparatus, comprising:
- a suspension liquid feeder configured to send a suspension liquid containing a mononuclear cell through a first flow channel in a first direction:
- a trap connected to the suspension liquid feeder via the first flow channel and configured to trap the mononuclear cell contained in the suspension liquid sent through the first flow channel from the suspension liquid feeder, the trap being arranged in the first flow channel;
- and
- an inducing factor feeder, the inducing factor feeder being configured to send an inducing factor for inducing reprogramming of the mononuclear cell to the trap in a second direction opposite the first direction through the first flow channel, and to send a liquid containing the mononuclear cell and the inducing factor to a container, the container being connected to the trap via a second flow channel branching off from the first flow channel at a branch position upstream of the trap in the first direction.
Dependencies are inferred from the claim text.