Back to results
US 12,215,351 B2Granted
Methods for inducing hematopoietic stem cell specificity
AssigneeChildren's Medical Center Corporation
InventorsLeonard I. Zon, Joseph Mandelbaum
Life of this patent
Estimating the term…
Abstract
Described herein are methods for inducing HSC specification in a cell. Aspects of the invention relate to contacting a cell with a Runx1-CBFβ inhibitor for a specified period of time, and then removing the inhibitor from the cell. In some embodiments of any of the aspects, HSC specificity is maintained long-term.
Claims12
A method for inducing hematopoietic stem cell (HSC) specification in a cell, the method comprising;
- a) contacting a cell for a period of time with an agent selected from the group consisting of Ro5-3335, SU-5402, sc-221405, and AI-10;
- and
- b) removing said agent after said period of time, thereby inducing HSC specification in the cell,
- wherein the cell is selected from the group consisting of an hemogenic endothelial (HE) cell, an embryonic cell, an embryonic stem cell (ESC), a cell from a dissociated embryo, a cell from a dissociated embryo that is at least 9-hours post fertilization, an embryoid bodies, an induced pluripotent stem cell (iPSC), an aorta-gonad-mesonephros (AGM) cell, a placenta stem cell, an adult stem cell, and an amniotic stem cell,
- wherein the concentration of the agent is greater than 2 μM.
2 more elements
Dependencies are inferred from the claim text.