US 12,215,346 B2Granted

Method for isolating and culturing neural stem cells with high efficiency

AssigneeMedinno Inc.

InventorsKyeung Min Joo, Hyun Nam

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Abstract

The present invention relates to a method for isolating and culturing neural stem cells with high efficiency, which may shorten the time for isolation and culture by simplifying a method for isolating and culturing neural stem cells and may increase the acquisition yield of neural stem cells. The present invention provides a method for isolating and culturing neural stem cells with high efficiency, comprising the steps of adding brain tissue into an enzyme solution so as to subject the brain tissue to enzyme treatment; physically isolating cell clumps from the enzyme treated brain tissue by dividing the cell clumps according to size and removing impurities; and inoculating the cell clumps on a culture dish so as to subculture.

Claims3

Dependent claims

A method for isolating and culturing neural stem cells with high efficiency, comprising:

placing brain tissue in an enzyme solution for enzyme treatment;
physically dissociating cell clumps from the enzyme-treated brain tissue;
separating the cell clumps by size and removing impurities;
and
performing subculture by seeding the cell clumps that have a size greater than 70 μm and less than 100 μm in a culture dish,
wherein the method of isolating and culturing neural stem cells does not comprise isolating single cells,
wherein the step of separating the cell clumps by size comprises the steps of:
a) filtering the cell clumps through a 100 μm mesh and collecting the filtrate;
b) filtering the filtrate of step a) through a 70 μm mesh;
and
c) collecting the cell clumps caught by the 70 μm mesh.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/980,889Filed 30 Jan 2019
Priority: KR 10-2018-003110116 Mar 2018
Published: US 2022/0243175 A14 Aug 2022
Granted: 4 Feb 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 5/0619
Cells of the nervous system: neurons
C12N 5/0623
Cells of the nervous system: stem cells
C12N 9/226
Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes: class 2 CAS enzyme complex, e.g. single CAS protein
C12N 9/63
Proteinases , e.g. Endopeptidases: derived from plants

Family

US 2022/0243175 A1Pre-grant publication · published 4 Aug 2022
US 12,215,346 B2This patent

From the US filing history. Foreign family members aren’t listed.

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