Compositions and methods for the induction and tuning of gene expression
AssigneeDuke University
InventorsPablo Perez-Pinera, Charles A. Gersbach
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Abstract
Disclosed herein are compositions of transcription activator-like effectors transcription factors and methods of using the compositions for inducing gene expression of mammalian genes.
Claims8
A method of increasing gene expression of a target gene in a mammalian cell, the method comprising administering to the mammalian cell in vitro two or more transcription activator-like effector transcription factors (TALE-TFs) that bind to at least a first target region and a second target region in the target gene,
- wherein at least one of the first target region and the second target region is within a non-open chromatin region,
- wherein the first target region or the second target region comprises the nucleotide sequence of one of SEQ ID NOs:
- 1-28, or the complement thereof,
- wherein each TALE-TF consists essentially of a repeat variable diresidue, a transcription activation domain, and a nuclear localization sequence,
- wherein gene expression is increased in the mammalian cell, and
- wherein the gene expression is increased in the mammalian cell without the use of a chromatin modifying drug.
A method of increasing gene expression of a target gene in a mammalian cell, the method comprising administering to the mammalian cell in vitro two or more transcription activator-like effector transcription factors (TALE-TFs) that bind to at least a first target region and a second target region in the target gene,
- wherein at least one of the first target region and the second target region is within a non-open chromatin region,
- wherein each TALE-TF consists essentially of a repeat variable diresidue region, a transcription activation domain, and a nuclear localization sequence,
- wherein gene expression is increased in the mammalian cell,
- wherein the target gene is IL1RN, KLK3, CEACAM5, or ERBB2, and
- wherein the first target region or the second target region comprises a polynucleotide having a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1-28, or the complement thereof.
Dependencies are inferred from the claim text.