US 12,215,336 B2 · Cell-specific expression of modRNA

Abstract

Disclosed is an expression regulatory system for cell-specific transcription (expression) of a protein of interest, for example a cell cycle inducer that reactivates proliferation in adult or neonatal cardiomyocytes or insulin-producing beta cells. The expression regulatory system comprises a first nucleic acid that encodes a microRNA recognition element that specifically binds a target cell miR, and a translation suppressor protein; and a second nucleic acid that comprises a suppressor protein interaction motif that binds the translation suppressor protein, and a gene that encodes a protein of interest. When a cell of interest is co-transfected with the first and second nucleic acids of the system, the protein of interest expressed in a cell-specific fashion.

Claims20

Dependent claims

A method of expressing a protein of interest in a cell, comprising

contacting the cell with a first ribonucleic acid and a second ribonucleic acid, wherein
the first ribonucleic acid comprises a microRNA (miR) recognition element near its 3′UTR that specifically binds an miR expressed in the cell and encodes a translation suppressor protein, and
the second ribonucleic acid comprises a suppressor protein interaction motif that binds the translation suppressor protein and encodes the protein of interest;
wherein
one or both of
(a) the first ribonucleic acid comprises the nucleotide sequence of SEQ ID NO:
2, SEQ ID NO:
3, or SEQ ID NO:
4, and
(b) the second ribonucleic acid comprises the nucleotide sequence of SEQ ID NO:
5, SEQ ID NO:
6, SEQ ID NO:
7 or SEQ ID NO:
8.

11 more elements

Dependencies are inferred from the claim text.

Cell-specific expression of modRNA

Life of this patent

Cell-specific expression of modRNA

Life of this patent

Abstract

Claims20