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US 12,215,319 B2Granted
Methods and compositions for targeted genomic insertion
AssigneeSyngenta Crop Protection AG
InventorsZhongying Chen, Mary-Dell Chilton, Shujie Dong, Qiudeng Que, Heng Zhong
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Abstract
The present invention relates to methods and compositions for chimeric RNA comprising a guide RNA and bait RNA for modifying a target site in the genome of a cell. Such modifications include integration of a transgene and allelic mutations and modifications of native genes. Also provided are plants comprising a modified nucleic acid sequence compared to the native gene integrated into a targeted genomic site in the plant genome.
Claims9
A chimeric RNA for targeted genomic modification of a cell, wherein the chimeric RNA comprises:
- a) a guide RNA comprising a polynucleotide targeting guide sequence of at least 8 contiguous nucleotides and at least 80% identical to a target nucleic acid sequence of at least 8 contiguous nucleotides within a target nucleic acid molecule;
- and
- b) a bait RNA comprising at least 8 contiguous nucleic acids, wherein the nucleic acid sequence of the bait RNA is at least 70% complementary to at least 8 contiguous nucleic acids of a donor DNA molecule, wherein the donor DNA molecule is intended for integration into the genome of a cell, wherein a tracrRNA is operably linked to said bait RNA sequence.
Dependencies are inferred from the claim text.