Acetylated ribonucleic acids and uses thereof
AssigneeHelix Nanotechnologies Inc
InventorsNikhil Dhar, Kyle Backman, Dario de Jesus Davila Pasillas, Justin Sean Huang, Nikolai Eroshenko
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Abstract
Disclosed herein is a modified ribonucleotide comprising a nucleoside comprising 2′-O-acetylated ribose, and polyribonucleotides comprising the same. Also provided herein are compositions comprising a polyribonucleotide of the present disclosure and methods of making and using the same.
Claims26
A polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase,
- wherein the nucleobase is an adenine, a guanine, a cytosine, or an uracil, and
- wherein at least 80% of the ribose moieties of the polyribonucleotide are 2′-O-acetylated, and wherein the polyribonucleotide comprises a cap structure and the cap structure does not comprise a 2′-O-acetylated ribose.
A composition comprising a polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase,
- wherein the nucleobase is an adenine, a guanine, a cytosine, or an uracil, and wherein at least 80% of the ribose moieties of the polyribonucleotide are 2′-O-acetylated, and wherein the polyribonucleotide comprises a cap structure and the cap structure does not comprise a 2′-O-acetylated ribose.
A method comprising administering a polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase,
- wherein the nucleobase is an adenine, a guanine, a cytosine, or an uracil, and
- wherein at least 80% of the ribose moieties of the polyribonucleotide are 2′-O-acetylated, and wherein the polyribonucleotide comprises a cap structure and the cap structure does not comprise a 2′-O-acetylated ribose.
A polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase, wherein the nucleobase is N4-acetylcytosine, and
- wherein the polyribonucleotide comprises a cap structure and the cap structure does not comprise a 2′-O-acetylated ribose.
A method comprising administering a polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase, wherein the nucleobase is N4-acetylcytosine, and
- wherein the polyribonucleotide comprises a cap structure and the cap structure does not comprise a 2′-O-acetylated ribose.
A polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase, wherein the nucleobase is 5-hydroxymethyluracil.
A method comprising administering a polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase, wherein the nucleobase is 5-hydroxymethyluracil.
A polyribonucleotide comprising one or more modified ribonucleotides, wherein the one or more modified ribonucleotides comprise:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase, wherein the nucleobase is N1-methylpseudouracil.
A method of manufacturing an RNA composition comprising introducing at least one modified ribonucleotide into a polyribonucleotide, wherein the modified ribonucleotide comprises:
- (i) a 5′-monophosphate, and
- (ii) a nucleoside comprising a 2′-O-acetylated ribose and a nucleobase,
- wherein the nucleobase is an adenine, a guanine, a cytosine, or an uracil, and
- wherein at least 80% of the ribose moieties of the polyribonucleotide are 2′-O-acetylated.
Dependencies are inferred from the claim text.