Means and methods to modulate probiotic potency of the yeast Saccharomyces boulardii
AssigneesVib Vzw, Katholieke Universiteit Leuven
InventorsJohan Thevelein, Benjamin Offei, Maria Remedios Foulquié Moreno, Paul Vandecruys
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Abstract
Described herein are probiotics, more particularly [to] the probiotic yeast Saccharomyces boulardii. Even more particularly described herein are enhanced probiotic potency S. boulardii. Also described here are mutant alleles useful to develop yeast strains with enhanced production of acetic acid. In addition, described here is the use of such yeast strains for the production of dietary supplements or pharmaceutical compositions to improve gastrointestinal comfort.
Claims7
A Saccharomyces boulardii strain comprising a homozygous or hemizygous mutant WHI2 allele,
- wherein the mutant allele completely abolishes Whi2 function,
- wherein the wild type allele of Whi2 is SEQ ID NO:
- 3,
- wherein the mutant allele
- has been disrupted or deleted by homologous recombination or disrupted with a nuclease, and
- wherein the strain produces, at 37° C., a cell-free supernatant with a pH lower than 5.
A Saccharomyces boulardii strain comprising a heterologous WHI2 allele, wherein the allele completely abolishes Whi2 function.
Dependencies are inferred from the claim text.