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US 12,215,309 B2Granted
Identification of rare products of crossing organisms
AssigneeHeineken Supply Chain B.V.
InventorsArthur Roelof Gorter De Vries, Charlotte Catharina Koster, Jean-Marc Georges Daran, Jan-Maarten Geertman, Niels Gerard Adriaan Kuijpers
Life of this patent
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Abstract
The invention relates to methods for identifying a hybrid organism, based on staining of parent cells with a dye, preferably a fluorescent dye. A preferred dye is a succinimidyl ester-coupled dye. The invention further relates to the resulting hybrid organism, preferably an interspecies hybrid organism, that is labelled with a dye.
Claims10
A method for identifying a hybrid yeast organism, comprising
- (a) providing cells from a first and second yeast parent organism,
- whereby the first and second yeast parent organisms are mating-compatible,
- whereby the first yeast parent organism differs from the second yeast parent organism,
- whereby said first yeast parent organism, but not said second yeast parent organism, carries an auxotrophic marker,
- (b) labelling cells from the second yeast parent organism with a fluorescent dye;
- (c) hybridizing cells from the first yeast parent organism with labelled cells from the second yeast parent organism at a temperature between 5° C. and 15° C.;
- and
- (d) identifying a hybrid yeast organism as an auxotrophic, labelled cell.
5 more elements
A method for identifying a hybrid yeast organism, comprising
- (a) providing cells from a first and second yeast parent organism,
- whereby the first and second yeast parent organisms are mating-compatible,
- whereby the first yeast parent organism differs from the second yeast parent organism,
- whereby said first yeast parent organism, but not said second yeast parent organism, carries an auxotrophic marker,
- (b) labelling cells from the second yeast parent organism with a fluorescent dye;
- (c) hybridizing cells from the first yeast parent organism with labelled cells from the second yeast parent organism at a temperature that is at least 5° C. below an optimal growth temperature of the first yeast parent organism and/or the second yeast parent organism;
- and
- (d) identifying a hybrid yeast organism as an auxotrophic, labelled cell.
5 more elements
Dependencies are inferred from the claim text.