Bio-responsive adhesive antibody delivery platform for immunotherapy and preparation method thereof

A mussel adhesive protein (MAP)-antibody (MAP-Ab) conjugate comprising a MAP and an anti-cancer antibody,

• wherein the MAP is conjugated to the N-terminus of a peptide;
• wherein the anti-cancer antibody is conjugated to the C-terminus of the peptide;
• wherein the peptide is cleavable by a matrix metalloproteinase-2 (MMP2);
• wherein the peptide has the amino acid sequence set forth in SEQ ID NO:
• 12,
• wherein the anti-cancer antibody inhibits a surface protein of a cancer cell;
• wherein when the MAP-Ab conjugate is exposed to an MMP2, the anti-cancer antibody is released therefrom;
• and
• wherein the amino acid sequence of the MAP is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEO ID NO:
• 4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:
• 10, SEQ ID NO:
• 11, and any combination thereof.