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US 12,214,347 B2Granted
Nanopore/nanowell electrode enabled exonuclease sequencing
AssigneeElectronic Biosciences, Inc.
InventorsAnna E. P. Schibel, Eric N. Ervin, Sean German
Life of this patent
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Abstract
Devices and methods are provided for identifying individual monomeric units in sequential order as they are released or cleaved from a polymer strand via an enzyme, which acts on the polymer, and the monomeric units translocate through a transmembrane channel. Methods are also provided for identifying molecules as they translocate through a transmembrane channel.
Claims18
A method for determining a polymer sequence, comprising:
- a) electrophoretically and/or electroosmotically driving a polymer from a bulk solution through a transmembrane channel disposed in a membrane into a confined volume of a chamber;
- whereby the polymer contacts an enzyme in the confined volume of the chamber, under conditions in which the enzyme cleaves monomeric units from the polymer;
- b) electrophoretically and/or electroosmotically driving monomeric units in the order which they are sequentially cleaved from the polymer by the enzyme out of the confined volume of the chamber through the transmembrane channel;
- and
- c) determining the identity of each of the monomeric units based on its current signature, translocation time, and/or associated current noise level modulation as the monomeric unit translocates through the transmembrane channel, thereby determining the polymer sequence.
2 more elements
Dependencies are inferred from the claim text.