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AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsRobert E. Briggs, Fred M. Tatum
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The present invention relates to modified Mannheimia haemolytica (M. haemolytica) lktCA gene cluster cassettes, compositions comprising such cassettes, methods of using such cassettes and compositions, and kits comprising such cassettes and compositions. Also described herein are Mycoplasma bovis (M. bovis) protective antigens, compositions comprising such antigens, methods of using such antigens and compositions, and kits comprising such antigens and compositions. Also described herein are modified M. haemolytica lktCA gene cluster cassettes engineered to express M. bovis protective antigens, compositions comprising such cassettes, methods of using such cassettes and compositions, and kits comprising such cassettes and compositions.
A cassette comprising a modified Mannheimia haemolytica lktCA gene cluster, wherein the modified M. haemolytica lktCA gene cluster comprises two polynucleotides encoding leukotoxin neutralizing epitopes, a first polynucleotide encoding the native leukotoxin neutralizing epitope, and a second polynucleotide encoding an additional leukotoxin neutralizing epitope inserted immediately downstream of the native leukotoxin A start codon.
Dependencies are inferred from the claim text.