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US 12,213,997 B1Granted
Compositions and methods for supporting nucleus pulposus (NP) cell phenotype and biosynthesis
AssigneeWashington University
InventorsLori Setton, Marcos Barcellona, Julie Speer
Life of this patent
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Abstract
Among the various aspects of the present disclosure is the provision of compositions and methods for the generation of a supporting matrix for cells, such as stem cells and NP cells (and precursors thereof). For example, an NP cell-specific phenotype precursor cell can be an adult NP cell, such as a cell taken from the adult and degenerated intervertebral disc and/or a progenitor cell, such as stem cells, induced pluripotent cells, pluripotent cells, precursor cells, or stromal cells.
Claims42
A biocompatible composition comprising:
- a PEG maleimide biocompatible polymer component;
- and
- at least one cell-adhesive peptide,
- wherein
- the cell-adhesive peptide is between about 5 and about 30 amino acids long;
- the cell-adhesive peptide comprises at least one syndecan-binding or integrin-binding peptide selected from SEQ ID NO:
- 1, CGG-terminated SEQ ID NO:
- 1, SEQ ID NO:
- 2, and C-terminated SEQ ID NO:
- 2;
- the cell-adhesive peptide is coupled to the biocompatible polymer component to form a cell-adhesive peptide-functionalized monomer;
- the cell-adhesive peptide-functionalized monomer is crosslinked to form a cell-adhesive peptide-functionalized polymer, wherein the cell-adhesive peptide-functionalized polymer has a density greater than 10% (w/v);
- the biocompatible composition has a stiffness of about 10 kPa;
- and
- the biocompatible composition comprises a cell-adhesive peptide density between about 50 μM and about 100 μM sufficient to support nucleus pulposus (NP) cell-specific morphology, biosynthesis, or phenotype.
12 more elements
Dependencies are inferred from the claim text.