Back to results
US 12,213,730 B2Granted
Device and method for in vivo detection of clots within circulatory vessels
AssigneeBioVentures, LLC
InventorsVladimir Pavlovich Zharov, Ekaterina Galanzha
Life of this patent
Estimating the term…
Abstract
A device and method of using the device to detect the presence and composition of clots and other target objects in a circulatory vessel of a living subject is described. In particular, devices and methods of detecting the presence and composition of clots and other target objects in a circulatory vessel of a living subject using in vivo photoacoustic flow cytometry techniques is described.
Claims18
A method for continuous monitoring of a circulatory vessel of a living organism, the method comprising:
- pulsing circulating target objects comprising red blood cells and at least one clot within the circulatory vessel with at least one pulse of laser energy at a first pulse wavelength ranging between 400 nm and 2500 nm, wherein the first pulse wavelength induces a photoacoustic signal from the circulating target objects, wherein the at least one clot is a hemoglobin-rich red clot, a platelet-rich white clot, or a combination thereof and wherein the hemoglobin-rich clot is light absorbing and the platelet-rich white clot is non-absorbing;
- obtaining a photoacoustic pattern induced by the at least one pulse of laser energy, wherein the photoacoustic pattern comprises at least one photoacoustic signal comprising a blood background signal produced by the red blood cells;
- simultaneously obtaining fluorescence, scattering, and photothermal signals from the target objects, via a photodetector;
- processing photoacoustic, fluorescence, scattering, and photothermal signals;
- analyzing a combination of the photoacoustic pattern and the fluorescence, scattering, and/or photothermal signals to determine the presence of the at least one clot, wherein analyzing the photoacoustic pattern comprises determining the presence of positive and negative contrast peaks in the photoacoustic pattern indicating the presence of a red clot, a white clot, or a combination thereof, wherein the negative contrast in the photoacoustic pattern below the blood background signal indicates the presence of the white clot, the positive contrast in the photoacoustic pattern above the blood background signal indicates the presence of the red clot, and a combination of positive contrast and negative contrast indicates the presence of a combination of red and white clots;
- and
- producing a detection signal when the photoacoustic pattern and the fluorescence signal, scattering signal, photothermal signal, or combinations thereof indicates the presence of a clot.
4 more elements
A device for continuous monitoring of a circulatory vessel of a living organism, comprising:
- an in vivo flow cytometer, wherein the in vivo flow cytometer comprises:
- a pulsed laser configured to pulse target objects comprising light absorbing at least one hemoglobin rich red clot and/or at least one non-absorbing platelet-rich white clot within the circulatory vessel with a plurality of laser pulses at a pulse wavelength ranging between about 400 nm and about 2500 nm;
- an ultrasound transducer configured to receive photoacoustic signals emitted by the target objects in response to the laser pulses and generate an output;
- a photodetector configured to receive fluorescence, scattering, and/or photothermal signals emitted by the target objects;
- and
- a processor comprising an amplifier, a data recording system, and a data analysis system having data analysis software, the processor being configured to:
- receive the output from the ultrasound transducer and the fluorescence, scattering, and/or photothermal signals from the photodetector, wherein the output comprises a photoacoustic pattern;
- analyze a combination of the photoacoustic pattern, the fluorescence signal, the scattering signal, and/or the photothermal signal to determine the presence of the at least one red clot, wherein analyzing the photoacoustic pattern comprises determining the presence of positive and negative contrast peaks in the photoacoustic pattern, wherein the negative contrast in the photoacoustic pattern below a background photoacoustic signal indicates the presence of the white clot, the positive contrast in the photoacoustic pattern above the background photoacoustic signal indicates the presence of the red clot, and a combination of positive contrast and negative contrast indicates the presence of a combination of red and white clots;
- and
- produce a detection signal when the photoacoustic pattern and the fluorescence signal, scattering signal, photothermal signal, or combinations thereof indicates the presence of a clot.
7 more elements
A self-contained wearable device for continuous monitoring of a circulatory vessel of a living organism, comprising:
- an in vivo flow cytometer;
- a clot monitoring system configured to:
- emit a pulsed laser at target objects within the circulatory vessel;
- receive one or more signals comprising a photoacoustic pattern;
- analyze the photoacoustic pattern to determine a presence of at least one clot within the circulatory vessel based on a presence of positive and negative contrast peaks in the photoacoustic pattern wherein the negative contrast in the photoacoustic pattern below a blood background signal indicates the presence of a white clot, the positive contrast in the photoacoustic pattern above the blood background signal indicates the presence of a red clot, and a combination of positive contrast and negative contrast indicates the presence of a combination of red and white clots;
- and;
- produce a detection signal when the photoacoustic pattern indicates the presence of a clot;
- an alarm system to provide a warning to the living organism and/or summon medical attention;
- and
- a power source,
- wherein the in vivo flow cytometer, the clot monitoring system, the alarm system, and the power source are configured to be removably attachable to an appendage of the living organism using straps, adhesive patches, or adhesive strips, and wherein the appendage is chosen from a neck, wrist, finger, forearm, upper arm, hand, foot, ankle, lip, ear, chest, abdomen, eye head, scalp, or leg.
8 more elements
Dependencies are inferred from the claim text.