Systems and methods for prioritizing variants of unknown significance

A method comprising:

• processing a biological sample comprising a genetic isolate of a tumor biopsy or cheek swab from a patient with a cancer;
• generating, with a genetic sequencing device, a first genetic sequence in digital form using the biological sample, wherein the first genetic sequence contains a first variant of a gene;
• storing the first genetic sequence in a memory coupled with the genetic sequencing device, wherein the memory stores genetic sequences derived from multiple sequence datasets containing a plurality of variants of the gene;
• receiving the first genetic sequence from the memory;
• receiving a plurality of pathogenicity scores for the first variant of the gene, wherein each of the plurality of pathogenicity scores indicates a likely pathogenicity of the first variant, wherein at least one of the pathogenicity scores is a SIFT score, a Polyphen score, a likelihood ratio test (LRT) score, or a mutation taster score;
• calculating, with at least one processor, a prevalence of the first variant in a disease data set stored in a database accessible to the at least one processor;
• calculating, with the at least one processor, a prevalence of the first variant in a healthy population data set stored in the database;
• calculating, with the at least one processor, a mutation rate for the gene in the disease data set stored in the database;
• calculating, with the at least one processor, a mutation rate for the gene in the healthy population data set stored in the database;
• calculating, with the at least one processor, a zygosity of the first variant, wherein the zygosity is based at least in part on whether the first variant is homozygous or heterozygous;
• generating a first priority score for the first variant based, at least in part, on a combination of the plurality of pathogenicity scores, the calculated prevalences of the first variant in the disease data set and the healthy population data set, the calculated mutation rates for the gene in the disease data set and the healthy population data set, and the calculated zygosity of the first variant, wherein the first priority score indicates a significance of the first variant to the disease;
• calculating a plurality of priority scores for the plurality of variants, wherein each of the plurality of variants is associated with a different one of the plurality of priority scores;
• ranking the first variant among the plurality of variants to generate a ranking that includes the first variant and the plurality of variants, wherein the ranking is based on the first priority score for the first variant and the plurality of priority scores;
• applying a threshold priority score to the ranking;
• determining that the first priority score for the first variant is above the threshold priority score;
• displaying the first variant to a user on a display;
• determining a change in expression of a protein encoded by the gene based, at least in part, on the first variant;
• selecting a targeted therapy for treating the cancer based, at least in part, on the change in expression of the protein;
• and
• treating the cancer of the patient with the selected targeted therapy.