Methods of blood screening

A method of screening a blood sample obtained from a patient, wherein the patient has been administered an anti-CD38 antibody or antigen binding fragment thereof, the method comprising:

• a) providing a blood sample from the patient;
• b) providing a blood sample from a donor, wherein the donor blood sample comprises donor red blood cells;
• and
• c) screening the patient blood sample, comprising determining the presence or absence of one or more patient antibodies in the patient blood sample that specifically bind to one or more red blood cell antigens expressed on the surface of the donor red blood cells,
• wherein the method does not comprise a step of contacting the patient blood sample or the donor blood sample with an agent that inhibits binding of the anti-CD38 antibody or antigen binding fragment thereof to membrane-bound CD38 present on the surface of the one or more donor red blood cells,
• wherein anti-CD38 antibody or antigen binding fragment thereof comprises
• a) an HCDR1 comprising the amino acid sequence SEQ ID NO:
• 1;
• an HCDR2 comprising the amino acid sequence SEQ ID NO:
• 2;
• an HCDR3 comprising the amino acid sequence SEQ ID NO:
• 3;
• an LCDR1 comprising the amino acid sequence SEQ ID NO:
• 4;
• an LCDR2 comprising the amino acid sequence SEQ ID NO:
• 5;
• and
• an LCDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 6, 9, 10, 11 and 12;
• or
• b) an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 17;
• an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 18;
• an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 19;
• an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 20;
• an LCDR2 comprising the amino acid sequence of SEQ ID NO:
• 21;
• and
• an LCDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
• 22, 25 and 26.