Quantitation of insulin-like growth factor-I and insulin-like growth factor-II with high-resolution mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsCory Bystrom, Shijun Sheng, Nigel Clarke, Richard Reitz
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Abstract
Methods are provided for determining the amount of an IGF-I and/or IGF-II protein in a sample using high resolution/high accuracy mass spectrometry. The methods generally comprise enriching an IGF-I and/or IGF-II protein in a sample, ionizing an IGF-I and/or IGF-II protein from the sample to generate IGF-I and/or IGF-II protein ions, and determining the amount of IGF-I and/or IGF-II protein ions with high resolution/high accuracy mass spectrometry.
Claims24
A method for determining an amount of an insulin-like growth factor-II (IGF-II) protein in a serum or plasma sample, the method comprising:
- subjecting IGF-II protein in the sample to ionization by electron ionization, chemical ionization, electrospray ionization, photon ionization, atmospheric pressure chemical ionization, photoionization, atmospheric pressure photoionization, fast atom bombardment, liquid secondary ionization, matrix assisted laser desorption ionization, field ionization, field desorption, thermospray/plasmaspray ionization, surface enhanced laser desorption ionization, inductively coupled plasma, or particle beam ionization to produce one or more IGF-II ions detectable by mass spectrometry;
- and
- determining an amount of the one or more IGF-II ions by high resolution/high accuracy mass spectrometry, wherein the amount of the determined one or more IGF-II ions is related to the amount of the IGF-II protein in the sample, wherein:
- the one or more IGF-II ions detectable by mass spectrometry are selected from the group consisting of IGF-II ions with mass to charge ratios of 934.69±2, 1068.07±2, 1245.92±2, and 1494.89±2, or
- the one or more IGF-II ions detectable by mass spectrometry are selected from the group consisting of IGF-II ions with mass to charge ratios of 934.69±1, 1068.07±1, 1245.92±1, and 1494.89±1, or
- the one or more IGF-II ions detectable by mass spectrometry are selected from the group consisting of IGF-II ions with mass to charge ratios of 1067.36±0.1, 1067.51±0.1, 1067.65±0.1, 1067.80±0.1, 1067.94±0.1, 1068.08±0.1, 1068.23±0.1, 1068.37±0.1, 1068.51±0.1, 1068.65±0.1, 1068.80±0.1, 1068.94±0.1, and 1069.08±0.1.
A method for determining an amount of an insulin-like growth factor-II (IGF-II) protein in a serum or plasma sample, the method comprising:
- subjecting IGF-II protein in the sample to ionization by electron ionization, chemical ionization, electrospray ionization, photon ionization, atmospheric pressure chemical ionization, photoionization, atmospheric pressure photoionization, fast atom bombardment, liquid secondary ionization, matrix assisted laser desorption ionization, field ionization, field desorption, thermospray/plasmaspray ionization, surface enhanced laser desorption ionization, inductively coupled plasma, or particle beam ionization to produce one or more IGF-II ions detectable by mass spectrometry;
- and
- determining an amount of the one or more IGF-II ions by high resolution/high accuracy mass spectrometry;
- wherein the amount of the determined one or more IGF-II ions is related to the amount of the IGF-II protein in the sample;
- and
- the one or more IGF-II ions detectable by mass spectrometry are selected from the group consisting of IGF-II ions with mass to charge ratios of 1067.94±0.1 and 1068.08±0.1.
Dependencies are inferred from the claim text.