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US 12,181,480 B2Granted
Polyspecificity reagents, methods for their preparation and use
AssigneeAdimab, LLC
InventorsPiotr Bobrowicz, Amber D. Hanna, Jerry M. Thomas
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Abstract
The present invention relates, inter alia, to polyspecificity reagents, methods of making the same, and methods of using the same in, inter alia, the selection, screening, enrichment, and identification of non-polyspecific, and thus developable, polypeptides.
Claims11
A method of generating an enriched plurality of developable polypeptides from a non-enriched plurality of polypeptides, the method comprising:
- (a) providing the plurality of polypeptides;
- (b) contacting the plurality of step (a) with a polyspecificity reagent, wherein the polyspecificity reagent comprises a composition of:
- (i) a soluble cellular fraction;
- and,
- (ii) a membrane cellular fraction;
- thereby producing a reagent-plurality mixture;
- (c) detecting from the reagent-plurality mixture of step (b) one or more polyspecific polypeptides from among the plurality of polypeptides that interacts with the polyspecificity reagent;
- and
- (d) separating from the non-enriched plurality of polypeptides the one or more polyspecific polypeptides detected in step (c), thereby generating the enriched collection of developable polypeptides.
6 more elements
Dependencies are inferred from the claim text.