Reagents for detection of chimeric antigen receptor cells

Abstract

The invention is directed to a method for preparing a detection reagent for CAR expressing cells according to general formula (I) (X-S)m-D  (I) wherein D is a detection moiety, S a spacer unit and X a polypeptide comprising 10 to 80 amino acids and a N3 group, which binds to the antigen binding domain of a chimeric antigen receptor of a cell and m=4-200, characterized in reacting p detection moieties D with a spacer S according to general formula (II) wherein R is a leaving group, q=1 to 5, and n=4 to 200 and p is 4-250 thereby forming modified detection moiety Sp-D and reacting the modified detection moiety Sp-D with a plurality of polypeptides X to yield (X-S)m-D.

Dependencies are inferred from the claim text.