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US 12,181,462 B2Granted
Identification of viable human embryos
AssigneeOverture Life, Inc.
InventorsJosé A. Horcajadas, Hussain A. Abdulla
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a method for assessing the probability of embryo implantation success during the in vitro fertilization process. A method of the disclosure can predict embryo implantation success based on, for example, the amount of specific metabolites present in embryo conditioned culture media.
Claims44
A method comprising:
- a) obtaining a sample of culture media in which an embryo has been cultured in vitro;
- b) i) measuring from the sample an amount of one or more non-pregnancy metabolites associated with a low probability of embryo implantation success,
- wherein at least one of the non-pregnancy metabolites is indole, leucoline, skatole, 6-hydroxycaproic acid, 1,2,6-hexanetriol, naphthalen-2-amine, 8-hydroxyquinoline, 2,2,6,6-tetramethyl-4-piperidinol, 1,5-naphthalenediamine, 1H-indole-2-carboxylic acid, (E)-dacarbazine, dinitrosopentamethylenetetramine, 5-hydroxyindole-3-acetic acid, 3-amino-5,7-dimethyl-1-adamantanol, 11-aminoundecanoic acid, kynurenine, N-hydroxy-L-tryptophan, 2-(methylamino)-1H-benzo[de]isoquinoline-1,3 (2H)-dione, 5-hydroxy-N-formylkynurenine, or N-lauroylglycine;
- b) ii) measuring from the sample an amount of one or more pregnancy metabolites associated with a high probability of implantation success,
- wherein at least one of the pregnancy metabolites is caprolactam, N-cyclohexylformamide, threo-3-Hydroxy-2-methylbutyric acid, sulfoacetic acid, phenylalanine, (2,7-Dimethyloctahydro-1H-cyclopenta[c]pyridin-4-yl)methanol, trans-3-indoleacrylic acid, DL-tryptophan, pantothenic acid, diaveridine, (5-benzyl-3,6-dioxo-2-piperazinyl)acetic acid, or dasytrichone;
- c) comparing the amount of the one or more non-pregnancy metabolites and the amount of the one or more pregnancy metabolites;
- and
- d) transferring the one or more embryos with a high probability of implantation success based on step c) into a subject's uterus.
5 more elements
A method comprising:
- a) obtaining a sample of culture media in which an embryo has been cultured in vitro;
- b) i) measuring from the sample an amount of one or more non-pregnancy metabolites associated with a low probability of embryo implantation success,
- wherein at least one of the non-pregnancy metabolites is indole, leucoline, skatole, 6-hydroxycaproic acid, 1,2,6-hexanetriol, naphthalen-2-amine, 8-hydroxyquinoline, 2,2,6,6-tetramethyl-4-piperidinol, 1,5-naphthalenediamine, 1H-indole-2-carboxylic acid, (E)-dacarbazine, dinitrosopentamethylenetetramine, 5-hydroxyindole-3-acetic acid, 3-amino-5,7-dimethyl-1-adamantanol, 11-aminoundecanoic acid, N-hydroxy-L-tryptophan, 2-(methylamino)-1H-benzo[de]isoquinoline-1,3 (2H)-dione, 5-hydroxy-N-formylkynurenine, or N-lauroylglycine;
- b) ii) measuring from the sample an amount of one or more pregnancy metabolites associated with a high probability of implantation success,
- wherein at least one of the pregnancy metabolites is caprolactam, N-cyclohexylformamide, threo-3-Hydroxy-2-methylbutyric acid, sulfoacetic acid, phenylalanine, (2,7-Dimethyloctahydro-1H-cyclopenta[c]pyridin-4-yl)methanol, trans-3-indoleacrylic acid, DL-tryptophan, pantothenic acid, diaveridine, (5-benzyl-3,6-dioxo-2-piperazinyl)acetic acid, or dasytrichone;
- c) comparing the amount of the one or more non-pregnancy metabolites and the amount of the one or more pregnancy metabolites;
- and
- d) transferring the one or more embryos with a high probability of implantation success based on step c) into a subject's uterus.
5 more elements
A method comprising:
- a) obtaining a sample of culture media in which an embryo has been cultured in vitro;
- b) i) measuring from the sample an amount of one or more non-pregnancy metabolites associated with a low probability of embryo implantation success,
- wherein at least one of the non-pregnancy metabolites exhibits a molecular weight of about:
- 117.06, 129.06, 131.07, 132.08, 134.09, 143.07, 145.05, 157.15, 158.08, 161.05, 182.09, 186.09, 191.06, 195.16, 201.17, 208.08, 220.08, 226.07, 252.07, or 257.20;
- b) ii) measuring from the sample an amount of one or more pregnancy metabolites associated with a high probability of implantation success,
- wherein at least one of the pregnancy metabolites exhibits a molecular weight of about:
- 113.08, 127.10, 132.08, 139.94, 165.08, 183.16, 187.06, 204.09, 219.11, 260.12, 262.09, or 296.10;
- c) comparing the amount of the one or more non-pregnancy metabolites and the amount of the one or more pregnancy metabolites;
- and
- d) transferring the one or more embryos with a high probability of implantation success based on step c) into a subject's uterus.
7 more elements
Dependencies are inferred from the claim text.