Chromatography column positioning assemblies

A chromatography column positioning assembly for accommodating different chromatography column lengths, comprising:

• an inlet face seal;
• an outlet face seal;
• a chromatography column holder of a first length for holding a chromatography column in a position for creating a fluidic connection, the chromatography column holder:
• being configured for multiple uses with chromatography columns of different lengths,
• having a length to engage with the inlet face seal and the outlet face seal and which is a length of a longest chromatography column for which the chromatography column holder is configured for use with;
• and
• an extender for positioning in the chromatography column holder to occupy at least a portion of the first length of the chromatography column holder that is not occupied when the chromatography column holder holds a chromatography column of a second length, where the second length is less than the first length.