Method for the quick identification of microorganisms by analysis of excitation-emission matrices
AssigneesUniversite Du Mans, Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire D'Angers, Universite D'Angers
InventorsJean-François Bardeau, Jean-Philippe Bouchara
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Abstract
A method for identifying a microorganism to be identified, which includes the following steps: obtaining an Excitation-Emission EEM of the microorganism to be identified, analysing the main components of the EEM matrix using at least one reference EEMr matrix, projecting the result of the analysis onto a plane defined by two main components, and identifying the microorganism to be identified.
Claims10
A method for identifying a microorganism to be identified comprising the following steps:
- obtaining an Excitation-Emission matrix (EEM) of the microorganism to be identified,
- analysing the main components of said EEM matrix using at least one reference Excitation-Emission matrix (EEMr), where the analysing comprises:
- /i/unfolding the EMM matrix of the microorganism to be identified after obtaining said EMM matrix into a single characteristic vector of the microorganism;
- and
- /ii/analysing the main components of a data matrix, said data matrix comprising said vector obtained in the preceding step using at least one other vector (vr) coming from the unfolding of at least one EEMr matrix of a reference microorganism, the at least one EEMr matrix being obtained in the same conditions as the EEM matrix,
- projecting the result of the analysis onto a plane defined by two main components, and
- identifying the microorganism to be identified,
- where obtaining the EEM matrix of the microorganism to be identified is carried out with a fluorometer.
Dependencies are inferred from the claim text.