Systems and methods for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration

An ultraviolet (UV) based imaging method for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration, the UV based imaging method comprising:

• projecting a multi-wavelength light beam toward a monochromator;
• projecting a range of single-wavelength light beams based on the multi-wavelength light beam toward a protein sample, wherein the protein sample includes either an unknown protein sample or a standard protein sample;
• detecting a range of single-wavelength light beams that pass through at least a portion of the protein sample;
• recording the range of single-wavelength light beams as wavelength data;
• receiving, at a processor, a standard set of wavelength data as recorded by a detector for each standard protein sample of a set of standard protein samples, wherein the standard set of wavelength data includes, for each standard protein sample, a first series of first absorbance-to-wavelength value pairs across a first range of wavelengths selected from a range of a single-wavelength light beams of a UV spectra, each first absorbance-to-wavelength value pair comprising a UV absorbance value and a wavelength value;
• generating, with the processor, a multi-wavelength calibration model based on each of the first series of first absorbance-to-wavelength value pairs of the standard set of wavelength data;
• receiving, at the processor, an unknown set of wavelength data as recorded by the detector for each unknown protein sample of a set of unknown protein samples, wherein the unknown set of wavelength data includes, for each unknown protein sample, a second series of second absorbance-to-wavelength value pairs across a second range of wavelengths selected from the range of the single-wavelength light beams, each second absorbance-to-wavelength value pair comprising a UV absorbance value and a wavelength value;
• and
• determining, with the multi-wavelength calibration model as implemented on the processor, for each unknown protein sample of the set of unknown protein samples, a plurality of protein concentration values, each protein concentration value of the plurality of protein concentration values corresponding to the second range of wavelengths selected from the range of the single-wavelength light beams.

A tangible, non-transitory computer-readable medium storing instructions for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration, that when executed by one or more processors of a computing device, cause the computing device to:

• receive, at a processor, a standard set of wavelength data as recorded by a detector for each standard protein sample of a set of standard protein samples, wherein the standard set of wavelength data includes, for each standard protein sample, a first series of first absorbance-to-wavelength value pairs across a first range of wavelengths selected from a range of a single-wavelength light beams of a UV spectra, each first absorbance-to-wavelength value pair comprising a UV absorbance value and a wavelength value;
• generate, with the processor, a multi-wavelength calibration model based on each of the first series of first absorbance-to-wavelength value pairs of the standard set of wavelength data;
• receive, at the processor, an unknown set of wavelength data as recorded by the detector for each unknown protein sample of a set of unknown protein samples, wherein the unknown set of wavelength data includes, for each unknown protein sample, a second series of second absorbance-to-wavelength value pairs across a second range of wavelengths selected from the range of the single-wavelength light beams, each second absorbance-to-wavelength value pair comprising a UV absorbance value and a wavelength value;
• and
• determine, with the multi-wavelength calibration model as implemented on the processor, for each unknown protein sample of the set of unknown protein samples, a plurality of protein concentration values, each protein concentration value of the plurality of protein concentration values corresponding to the second range of wavelengths selected from the range of the single-wavelength light beams.