System and method for sample preservation
AssigneeVentana Medical Systems, Inc.
InventorsDaniel Bauer, David Chafin, Michael Otter
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Abstract
A method is disclosed that permits calculation of reagent concentrations (in SI units) over time and space within a tissue sample as the sample is immersed in the reagent and the reagent diffuses into the tissue sample. The disclosed method has yielded the surprising result that once a formaldehyde concentration at all points within a tissue sample exceeds about 90 mM during a cold step of a cold+hot fixation protocol, the hot step of the fixation protocol can be commenced to provide reliable detection of molecular targets and preservation of tissue morphology in downstream analyses.
Claims5
A method for fixing a tissue sample, comprising:
- immersing the tissue sample in a cold formalin solution until a point at the center of the tissue sample is determined to have a formaldehyde concentration of at least 90 mM, wherein the immersing of the tissue sample in the cold formalin solution is performed under conditions sufficient to diffuse the formaldehyde to the center point of the tissue sample;
- and
- contacting the tissue sample with a hot formalin solution once formaldehyde concentration at the point at the center of the tissue sample is determined to be above 90 mM for a time sufficient to form cross-links, thereby fixing the tissue sample;
- wherein the cold formalin solution has a temperature of between about 0° C. and about 15° C., and
- wherein the hot formalin solution has a temperature of between about 20° C. and about 55° C.
Dependencies are inferred from the claim text.