Lipid-preserving refractive index matching for prolonged imaging depth for transparent tissue sample and composition
AssigneesCase Western Reserve University, University Hospitals Cleveland Medical Center, Vanderbilt University
InventorsMichael W. Jenkins, Yehe Liu
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Abstract
The present disclosure relates to an optical clearing method called lipid-preserving index matching for prolonged imaging depth (LIMPID). The optical clearing method can include simply immersing a sample in an optical clearing solution before imaging (e.g., with microscopy or optical coherence tomography). The optical clearing solution can include water; an iodine-containing non-ionic radiocontrast agent; and urea.
Claims13
An optical clearing solution for a labeled sample, the optical clearing solution comprising:
- water;
- an iodine-containing, non-ionic radiocontrast agent comprising 40 to 80 weight percent iodixanol in solution with 1 to 20 weight percent triethanolamine;
- 1 to 20 weight percent of iohexol;
- and
- urea for lipid preservation, wherein the urea has a concentration of 10 to 50 weight percent,
- wherein the optical clearing solution is configured to preserve lipids within the labeled sample without reducing diffusion speed through a synergistic combination of at least the iodine-containing, non-ionic radiocontrast agent comprising the 40 to 80 weight percent iodixanol in solution with the 1 to 20 weight percent triethanolamine and the urea having the concentration of 10 to 50 weight percent.
A method comprising:
- immersing a labeled sample in an optical clearing solution before imaging,
- wherein the optical clearing solution comprises:
- water;
- an iodine-containing, non-ionic radiocontrast agent comprising 40 to 80 weight percent iodixanol in solution with 1 to 20 weight percent triethanolamine;
- 1 to 20 weight percent of iohexol;
- and
- urea to preserve lipids of a labeled sample, wherein the urea has a concentration of 10 to 50 weight percent;
- and
- imaging the labeled sample with lipids within the labeled sample preserved without reducing diffusion speed.
An optical clearing solution for a labeled sample, the optical clearing solution consisting of:
- water;
- an iodine-containing, non-ionic radiocontrast agent comprising 40 to 80 weight percent iodixanol in solution with 1 to 20 weight percent triethanolamine;
- 1 to 20 weight percent of iohexol;
- and
- urea for lipid preservation, wherein the urea has a concentration of 10 to 50 weight percent,
- wherein the optical clearing solution is configured to preserve lipids within the labeled sample without reducing diffusion speed through a synergistic combination of at least the iodine-containing, non-ionic radiocontrast agent comprising the 40 to 80 weight percent iodixanol in solution with the 1 to 20 weight percent triethanolamine and the urea having the concentration of 10 to 50 weight percent.
Dependencies are inferred from the claim text.