Method and device for detecting at least one microorganism according to the staining kinetics thereof, and detection support

A method for detecting, on a solid detection support, at least one microorganism, of bacteria, yeast or mould type, present in a sample to be analysed and revealed by means of at least one cell marker, said method comprising at least the steps, in a sequential order, of:

• a1) depositing said sample to be analysed on said solid detection support;
• b1) illuminating locally said detection support by light radiation capable of revealing said at least one cell marker;
• c1) simultaneously with the illumination step b1), acquiring at least one image (I0, I′0) in a first phase P0 of said sample on at least one portion of said detection support by means of an optical imaging device targeting a field of view that is at least 10 mm long by at least 10 mm wide;
• d1) carrying out a controlled release of said at least one cell marker, through the detection support, to bring said marker into contact with said microorganism;
• e1) within a lapse of time of less than 5 seconds after step d1) of the controlled release of said cell marker, illuminating locally said detection support with said light radiation capable of revealing said at least one cell marker;
• f1) simultaneously with the illumination step e1), acquiring an image (I1, I′1), by means of said optical device, of each portion of the detection support of which an image was acquired at P0 in at least one subsequent phase P1 after the step of the controlled release of said marker;
• g1) re-illuminating locally, at least once, said detection support with said light radiation;
• h1) simultaneously with the illumination step g1), acquiring an image (I2, I′2), by means of said optical device, of each portion of the detection support of which an image was acquired at P0 and at P1 in at least one second subsequent phase P2 in order to detect said at least one microorganism according to the change in the staining kinetics of said microorganism;
• and
• i1) carrying out a comparative analysis, per portion of the detection support, between said image (I0, I0) acquired at P0 and said images (Ii, I′1;
• I2, I′2) acquired in at least two subsequent phases P1 and P2 and drawing a conclusion as regards the presence or absence of at least one microorganism in said sample.