Back to results
US 12,180,518 B2Granted
RNA polymerase variants for co-transcriptional capping
AssigneeModernaTX, Inc.
InventorsAthanasios Dousis, Kanchana Ravichandran, Amy E. Rabideau, Margaret Franklin, Kevin Smith, Michelle Lynn Hall
Life of this patent
Estimating the term…
Abstract
The present disclosure provides RNA polymerase variants for high efficiency transcription.
Claims20
A method comprising:
- incubating a deoxyribonucleic acid (DNA) in an in vitro transcription reaction that comprises nucleoside triphosphates, buffer, and a ribonucleic acid (RNA) polymerase variant, wherein the RNA polymerase variant (i) comprises an amino acid substitution at position D351 relative to an RNA polymerase comprising the amino acid sequence of SEQ ID NO:
- 1 and (ii) has at least 95% identity to the amino acid sequence of SEQ ID NO:
- 1, thereby producing a messenger RNA (mRNA).
Dependencies are inferred from the claim text.