Methods for reprograming non-pluripotent cells into pluripotent stem cells

A method of inducing pluripotency in partially or completely differentiated cells, the method comprising:

• (a) contacting the partially or completely differentiated cells with a first cocktail comprising VC6TFAE (XEN-Cocktail) for a sufficient period of time to obtain XEN-like cells;
• (b) contacting the XEN-like cells for a sufficient period of time to reprogram the XEN-like cells into chemically induced pluripotent stem cells (CiPSCs) with a second cocktail comprising VC6TFZASD (XEN-CiPSC cocktail);
• and
• (c) culturing the CiPSCs in 2i-medium,
• wherein V is valproic acid,
• C is [6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile] (CHIR99021),
• 6 is [2-(3-(6-Methylpyridin-2-yl)-1H-pyrazol-4-yl)-1,5-naphthyridine] (616452),
• T is tranylcypromine,
• F is Forskolin,
• A is (4-[(5,6,7,8-Tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl) carboxamido]benzoic acid) (AM-580),
• E is 1-(3-((((2R,3S,4R,5R)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl) (isopropyl)amino) propyl)-3-(4-(tert-butyl)phenyl) urea (EPZ004777),
• Z is 3-deazaneplanocin A (DZNep),
• S is (1-[3-[[[(2R,3S,4R,5R)-5-(4-Amino-5-bromo-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl](isopropyl)amino]propyl]-3-[4-(2,2-dimethylethyl)phenyl]urea) (SGC 0946), and
• D is 5-azacytidine.