Constructs and methods for controlling stomatal closure in plants
AssigneesLimagrain Europe S.A., Commissariat A L'energie Atomique Et Aux Energies Alternatives
InventorsNathalie Leonhardt, Ludovic Martin, Serge Chiarenza, Helene Jacquet, Laurent Nussaume, Marie Javelle
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Abstract
The present invention is related to expressing an AHA5 protein in plants, and preferentially a mutated AHA5 protein leading to a constitutive activity of AHA5, to control stomatal closure and improve tolerance to drought conditions and yield in plants.
Claims7
A method for decreasing water loss in a plant comprising introducing a genetic mutation into a plasma membrane H+-ATPase 5 (AHA5) gene of Arabidopsis or Zea Mays to generate a gene with a genetic mutation, wherein the gene with the genetic mutation encodes a mutated AHA5 protein, in a cell of the plant with gene editing techniques,
- wherein said mutated AHA5 protein is selected from:
- (a) a mutated AHA5 protein comprising the amino acid sequence of SEQ ID NO:
- 31;
- (b) a mutated AHA5 protein comprising the amino acid sequence of SEQ ID NO:
- 30;
- and
- (c) a mutated AHA5 protein comprising the amino acid sequence of SEQ ID NO:
- 23;
- and
- regenerating said cell to obtain a whole plant, wherein the mutated AHA5 protein is at least expressed in guard cells and wherein said plant has decreased water loss.
Dependencies are inferred from the claim text.