Methods and systems for producing a protein of interest in a plant
AssigneeImmunityBio, Inc.
InventorPatrick Soon-Shiong
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Abstract
Methods and systems for producing a protein of interest within a plant or a portion of a plant are provided herein. The method includes introducing one or more nucleic acid into the plant or the portion of the plant, the nucleic acid including a nucleotide sequence encoding the protein of interest and incubating the plant or the portion of the plant under conditions that permit the expression of the nucleotide sequence encoding the protein of interest. The method also includes adding a medium amendment including a calcium carbonate source, such as aragonite, to a medium environment of the plant.
Claims20
A method for producing a protein of interest within a plant or a portion of a plant comprising:
- a) introducing one or more nucleic acids into the plant or the portion of the plant, the nucleic acid comprising a nucleotide sequence encoding the protein of interest and operatively linked to a regulatory region;
- b) incubating the plant or the portion of the plant under conditions that permit expression of the nucleotide sequence encoding the protein of interest, thereby producing the protein of interest;
- and
- c) adding a medium amendment to a medium environment of the plant, wherein the medium amendment comprises a calcium carbonate source comprising aragonite,
- wherein the protein of interest is nogapendekin-alfa-inbakicept (NAI), a heterodimeric bifunctional fusion complex comprising a soluble TGF-BRII domain and an IL-15/IL15RαSu domain linked by a tissue factor linker, a T×M, or a mAb;
- and
- wherein the plant is from a genus selected from the group consisting of Arabidopsis, Nicotiana, Brassica, Ipomoea, Zea, Sorghum, Carthamus, Glycine, Triticum, Solanum, Avena, Secale, Medicago, Helianthus, Gossypium, Hordeum, Oryza, Panax, and Pisum.
Dependencies are inferred from the claim text.