Method for detecting CD4+CD25+glycoprotein A33(GPA33)high and/or CD4+CD127-GPA33high T cells
AssigneesStichting Sanquin Bloedvoorziening, Stichting Het Nederlands Kanker Institmit-Antoni van Leeuwenhoek Ziekenhuis
InventorsDerk Amsen, Eloy Cuadrado Godia, Jannetje Geertruida Borst
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Abstract
The invention relates to isolated populations of CD4+GPA33high regulatory T cells, to methods for detecting and/or isolating CD4+GPA33high cells, methods for enriching for CD4+GPA33high cells and to uses of CD4+GPA33high cells and populations of such cells. The invention further relates to methods for classifying an individual suffering from cancer and typing a tumor sample as well as for determining the etiology of autoimmune diseases.
Claims10
A method for detecting CD4+CD25+ glycoprotein A33(GPA33)high and/or CD4+CD127−GPA33high T cells in a sample comprising:
- obtaining a cell sample;
- and
- detecting CD4+CD25+GPA33high and/or CD4+CD127−GPA33high T cells by contacting the cell sample with an anti-CD4 antibody or antigen-binding fragment thereof and an anti-GPA33 antibody or antigen-binding fragment thereof and detecting binding between cells in the sample and the anti-CD4 antibody or antigen-binding fragment thereof and the anti-GPA33 antibody or antigen-binding fragment thereof, and contacting the cell sample with an anti-CD25 antibody or antigen-binding fragment thereof and detecting binding between cells in the sample and the anti-CD25 antibody or antigen-binding fragment thereof to detect CD4+CD25+GPA33high T cells, and/or contacting the cell sample with an anti-CD127 antibody or antigen-binding fragment thereof and detecting binding or the lack thereof between cells in the sample and the anti-CD127 antibody or antigen-binding fragment thereof to detect CD4+CD127−GPA33high T cells,
- wherein GPA33high is defined as the expression level of GPA33 that is higher than the average level of expression of GPA33 on CD4+ T cells in the cell sample.
A method for detecting CD4+CD25+ glycoprotein A33(GPA33)high and/or CD4+CD127−GPA33high T cells in a sample, comprising:
- obtaining a cell sample;
- and
- detecting CD4+CD25+GPA33high and/or CD4+CD127−GPA33high T cells by contacting the cell sample with an anti-CD4 antibody or antigen-binding fragment thereof and an anti-GPA33 antibody or antigen-binding fragment thereof and detecting binding between cells in the sample and the anti-CD4 antibody or antigen-binding fragment thereof and the anti-GPA33 antibody or antigen-binding fragment thereof, and contacting the cell sample with an anti-CD25 antibody or antigen-binding fragment thereof and detecting binding between cells in the sample and the anti-CD25 antibody or antigen-binding fragment thereof to detect CD4+CD25+GPA33high T cells, and/or contacting the cell sample with an anti-CD127 antibody or antigen-binding fragment thereof and detecting binding or the lack thereof between cells in the sample and the anti-CD127 antibody or antigen-binding fragment thereof to detect CD4+CD127−GPA33high T cells, wherein GPA33high is defined as the expression level of GPA33 at which 75% of CD4+CD25+CD45RA+ T cells are GPA33+.
Dependencies are inferred from the claim text.