Systems, devices, and methods for electroporation within a cell processing system

An electroporation chamber, comprising:

• a first electrode and a second electrode;
• a plurality of ports comprising
• an inlet port at a first portion of the electroporation chamber, and
• an outlet port at a second portion of the electroporation chamber;
• a fluid conduit arranged between the first electrode and the second electrode, the fluid conduit being in fluid communication with the inlet port, the outlet port, and each of a plurality of ports via auxiliary channels extending therefrom;
• and
• at least one window disposed on a wall of the electroporation chamber and providing a view to a segment of the fluid conduit,
• wherein the at least one window comprises a first window and a second window, the first window having a view to a first segment of the fluid conduit and the second window having a view to a second segment of the fluid conduit, and wherein the second window is proximate a first auxiliary channel of the plurality of auxiliary channels that is in fluid communication with the outlet port.